Two-dimensional gel electrophoresis of acid-extractable nuclear proteins of regenerating and thioacetamide-treated rat liver, Morris 9618a hepatoma, and Walker 256 carcinosarcoma.

The acid-soluble nuclear proteins of regenerating and thioacetamide-treated rat livers as well as the Morris 9618A hepatoma and the Walker 256 carcinosarcoma were ex tracted from citric acid-isolated nuclei with 0.4 N H2SO4. The nuclear extracts were analyzed by two-dimensional polyacrylamide gel electrophoresis. Although most of the protein spots were common to the livers and tumors stud ied, all of the rodent tumors were similar in their marked density of Spots C16-C18. In normal, regenerating, and thioacetamide-treated liver, the spots in this region were much less dense.